Fig. 1.

Influence of binding-affinity bias on information content. a Binding-affinity bias in the reference species. The left column shows binding sites bound in vivo as well as the sequence logo. In the right column, enrichment of high-affinity binding sites by chromatin immunoprecipitation leads to a different motif with higher information content. b Binding-affinity bias in the reference species and three phylogenetically related species. The left column shows binding sites bound in vivo and the information content of the species–specific motifs. In the right column, the enrichment of high-affinity binding sites in the reference species and the other three species leads to different motifs with different information content in each species. The effect of this enrichment decreases with the phylogenetic distance from the reference species as reflected by decreasing information contents. Please find the sequences of all species in Table 1