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. 2016 May 10;16:110. doi: 10.1186/s12870-016-0797-1

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© Barkla et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Visualization of EBC chloroplast autofluorescence by confocal laser scanning microscopy. Stem sections from salt-treated plants were submerged in water and images were obtained using an Olympus FV1000 confocal laser scanning microscope using an XLPLN 25X W NA:1.05 water immersion objective lens. Laser wavelength 1 = 488 (green) cell wall autofluorescence, Laser wavelength 2 = 635 (red) chloroplast autofluorescence. (a) Chloroplasts in EBC and (b), mesophyll cells. (c) Each panel is a single confocal section taken from a Z-stack of 12 confocal images acquired at 20 μm intervals