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. 2016 May 10;16:110. doi: 10.1186/s12870-016-0797-1

Table 1.

Results from DIGE analysis of control vs salt treated EBC extract

DIGE
Spot No. t-testa Av. Ratiob Protein(s) in Spotc GO locationd GO biological processe Uniprot # Species Signifcant
Unique
peptidesf 		M.W./pIg
664 0.00013 2.48 V-ATPase A subunit TP transport Q9AVU8 M. crystallinum 12 69/5.09
740-1 Nitrite reductase CP nitrogen metabolism B9RYH9 R. communis 4 66/6.04
9.70E-05 2.61
740-2 Beta-hexosaminidase 1 V carbohydrate
metabolism		 A7WM73 A. thaliana 3 61/5.88
760 0.00031 2.93 V-ATPase B subunit TP transport Q8GUB5 M. crystallinum 31 54/4.96
765 0.0002 2.68 NADP-dependent malic enzyme Cyt CAM P37223 M. crystallinum 46 64/6.06
767 0.00062 2.30 NADP-dependent malic enzyme Cyt CAM P37223 M. crystallinum 49 64/6.06
774 0.0033 1.67 NADP-dependent malic enzyme Cyt CAM P37223 M. crystallinum 48 64/6.06
775 0.0071 1.58 NADP-dependent malic enzyme Cyt CAM P37223 M. crystallinum 54 64/6.06
923 0.0016 −1.79 no proteins identified
1062 0.00053 −1.59 Alpha-1,4-glucan protein synthase AP cell wall metabolism I1J637 G. max 16 42/5.52
1064 0.0014 2.00 no proteins identified
1065 0.0026 1.96 Beta-D-galactosidase AP cell wall metabolism Q5CCQ1 P. pyrifolia 3 41/5.5
1071-1 2-phosphoglycerate hydrolase Cyt glycolysis Q43130 M. crystallinum 12 48/5.62
0.001 2.00
1071-2 Alpha-1,4-glucan protein Cyt cell wall metabolism I1J637 G. max 9 42/5.52
1095 0.0034 1.69 Malate dehydrogenase Cyt CAM Q645N1 S. lycopersicum 4 36/8.87
1123-1 Malate dehydrogenase Cyt CAM/ Q645N1 S. lycopersicum 5 36/8.87
0.00049 2.13
1123-2 Proline iminopeptidase Cyt proteolysis B9G1Q0 P. trichocarpa 4 37/8.96
1153 3.20E-07 6.20 V-ATPase E subunit TP transport Q40272 M.crystallinum 26 26/6.52
1290 0.032 −1.43 no proteins identified
1307 0.0019 2.20 Ascorbate peroxidase Cyt stress response C5J0H6 S. nigrum 5 18/4.83
1325 0.0082 −1.66 Fructose bisphosphate aldolase, Cyt glycolysis O04975 M. crystallinum 8 38/6.49
1501 2.40E-07 −2.51 Alpha-galactosidase-like protein AP cell wall metabolism D7LVE6 A. lyrata 3 48/4.79
1502 0.003 1.63 5-methyltetrahydropteroyltri-glutamate-homocysteine methyltransferase Cyt amino acid metabolism P93263 M. crystallinum 62 85/5.9
1503 0.03 −1.4 Triosephosphate isomerase Cyt glycolysis I3SN66 M. tribuloides 5 33/6.54
1508 4.40E-05 2.4 V-ATPase A subunit TP transport Q9AVU8 M. crystallinum 54 69/5.09

Data are from 4 independent biological experiments for each treatmentaStudent’s t-test p values are given as a measure of confidence for the ratio of each spot measured

bAverage ratios of spot abundance of salt-treated samples relative to the untreated control represent data from four independent experiments

cProtein names, dGO cellular location and eGO biological function annotations are taken from the Uniprot database recommended name and annotation

f p < 0.05; Peptide sequences, % sequence coverage, best Mascot ion, charge and delta ion score as well as spectra charge states can be seen for all identified proteins in Additional file 5

gTheoretical molecular weight and isoelectric point for each protein spot identified