Fig. 5.

Examples of hematoxylin and eosin staining of harmonic motion imaging for focused ultrasound-ablated normal breast (a–c), invasive ductal carcinoma (d–f), and fibroadenoma (g–i) tissues. In (a), (d), and (g), scale bars indicate 4 mm; in the rest of the images, scale bars indicate 0.1 mm. In (b), (e), and (h), high-magnification images display unablated regions taken within the corresponding black frames. In (c), (f), and (i) high-magnification images display ablated regions taken within the corresponding blue frames. The ablated regions show discrete hypereosinophilic areas reflecting changes occurring in the collagenous stroma as well as cautery-like “streaming” phenomena (arrows) in the HIFU-ablated epithelial regions. The changes seen in the epithelium and/or nuclei are (nonneoplastic, benign, or malignant) reminiscent of what is seen in tissue exposed to electrocautery change. Surrounding tissues remained histologically intact