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. 2016 May 10;7:627. doi: 10.3389/fpls.2016.00627

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Copyright © 2016 Bhattacharjee, Khurana and Jain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transactivation and dimerization properties of homeobox proteins. (A) Schematic representation of full-length homeobox proteins (BD::OsHOX24 and BD::OsHOX22) and truncated (C-terminal acidic region removed) fusion constructs (BD::OsHOX24ΔC and BD::OsHOX22ΔC) used. (B) Transactivation assay of full-length and truncated (ΔC) homeobox proteins in yeast. The transformants grown on SD-Trp (SD-T, left) medium; SD-Trp-His medium (SD-T-H, middle) and SD-Trp-His-Ade (SD-T-H-A, right) medium are shown. (C) Dimerization assay of full-length (fl) and truncated (ΔC) homeobox proteins. The deletion constructs, BD::OsHOX24ΔC and BD::OsHOX22ΔC, were co-transformed with different combinations of full-length (AD::OsHOX24fl, AD::OsHOX22fl) and deletion constructs (AD::OsHOX24ΔC, AD::OsHOX22ΔC) of homeobox proteins in yeast, as indicated on left side panel. The transformants were grown on SD-Trp-Leu (DDO medium) and SD-Trp-Leu-His-Ade medium (QDO medium) for confirmation of interaction. pGBKT7-p53 + pGADT7-T antigen represents positive control. Empty pGBKT7 vector (BD) represents negative control for transactivation assay and pGBKT7 + pGADT7 represents negative control for dimerization assay.