Table 1. Summary of colocalization between endogenous PCX and Rabs in 2D MDCK II cells.
| Rab | Time after plating | ||||
|---|---|---|---|---|---|
| 1 h | 3 h | 16 h | 24 h | 48 h | |
| Rab1A | − | − | − | − | − |
| Rab1B | − | − | − | − | − |
| Rab2A | − | − | − | − | − |
| Rab2B | − | − | − | − | − |
| Rab3A | − | − | − | +/− | +/− |
| Rab3B | − | − | − | + | +/− |
| Rab3C | +/− | +/− | + | + | +/− |
| Rab3D | +/− | +/− | + | + | +/− |
| Rab4A | +/− | +/− | +/− | − | − |
| Rab4B | +/− | +/− | +/− | +/− | − |
| Rab5A | ++ | ++ | + | - | − |
| Rab5B | ++ | ++ | ++ | + | − |
| Rab5C | ++ | ++ | ++ | + | − |
| Rab6A | − | − | − | − | − |
| Rab6B | − | − | − | − | − |
| Rab6C | ND | ND | ND | ND | ND |
| Rab7 | − | − | − | − | − |
| Rab8A | ++ | ++ | ++ | ++ | − |
| Rab8B | ++ | ++ | + | +/− | − |
| Rab9A | − | − | − | − | − |
| Rab9B | − | − | − | − | − |
| Rab10 | + | + | ++ | + | − |
| Rab11A | ++ | ++ | ++ | ++ | +/− |
| Rab11B | ++ | ++ | ++ | ++ | +/− |
| Rab12 | + | + | +/− | +/− | − |
| Rab13 | + | + | − | − | − |
| Rab14 | +/− | − | − | − | − |
| Rab15 | +/− | +/− | +/− | − | − |
| Rab17 | ++ | ++ | + | ++ | − |
| Rab18 | − | − | − | − | − |
| Rab19 | + | + | +/− | − | − |
| Rab20 | +/− | +/− | +/− | − | − |
| Rab21 | − | − | − | − | − |
| Rab22A | − | − | − | − | − |
| Rab22B | − | − | − | − | − |
| Rab23 | + | + | − | − | − |
| Rab24 | +/− | + | + | − | − |
| Rab25 | + | + | ++ | + | + |
| Rab26 | − | − | − | − | − |
| Rab27A | + | + | + | + | + |
| Rab27B | + | + | + | + | + |
| Rab28 | − | − | − | − | − |
| Rab29 | − | − | − | − | − |
| Rab30 | − | +/− | +/− | − | − |
| Rab32 | +/− | + | +/− | +/− | − |
| Rab33A | +/− | + | + | ++ | − |
| Rab33B | − | − | − | − | − |
| Rab34 | + | + | + | +/− | − |
| Rab35 | + | ++ | +/− | + | +/− |
| Rab36 | − | − | − | − | − |
| Rab37 | +/− | +/− | +/− | +/− | +/− |
| Rab38 | +/− | +/− | +/− | +/− | − |
| Rab39A | − | − | − | − | − |
| Rab39B | − | − | − | − | − |
| Rab40A | ND | ND | ND | ND | ND |
| Rab40B | ND | ND | ND | ND | ND |
| Rab40C | − | − | − | − | − |
| Rab41 | − | − | − | − | − |
| Rab42 | − | − | − | − | − |
| Rab43 | − | − | − | − | − |
Colocalization rate between PCX and EGFP-Rabs (or Myc-Rab40C) in 2D MDCK II cells is shown by ++ (very high colocalization), + (partial but clear colocalization), +/− (partial and less colocalization), and − (no colocalization). ND, not determined because of very low expression level of Rab6C, Rab40A, and Rab40B in MDCK II cells. Detailed image data for each Rab are shown in Figs. 3 and S3. The nomenclature of mammalian Rabs in this study is as described in Itoh et al. (2008).