Table 2. Summary of impaired PCX localization in Rab siRNA-treated 2D and 3D MDCK II cells.

siRNA	Defects observed in 3D cyst culture		Defects observed in 2D cell culture		Defects in polarized 2D cells
Rab3A	+	Intracellular retention of PCX near the apical site	+/−	PCX retention near cell–cell contact sites	−
Rab3B	+	Intracellular retention of PCX near the apical site	+/−	PCX retention near cell–cell contact sites	−
Rab3D	+	Intracellular retention of PCX near the apical site	+/−	PCX retention near cell–cell contact sites	−
Rab4A/B	+/−	Some multiple lumens	−		−
Rab5A	+	Some multiple lumens; reduced proliferation	+	Lateral lumens	+
Rab5B	+	PCX retention in large actin-rich vacuoles	+	Lateral lumens	+
Rab5C	−		−		−
Rab8A	+	Intracellular retention of PCX near the apical site	+	Intracellular retention of PCX	+
Rab10	−		−		−
Rab11A	+	Intracellular retention of PCX near the apical site/scattered vesicles	+	Delayed vesicle docking	−
Rab12	+	Retention of PCX in multiple large dots	+	Retention of PCX in multiple actin-free dots	−
Rab13	−		+	Intracellular retention of PCX	+
Rab14	−		+	Delayed vesicle internalization	−
Rab15	+	Intracellular retention of PCX	−	Increased spreading	−
Rab17	+	PCX retention in small intracellular vesicles	+	PCX retention near cell–cell contact sites	+
Rab19	+	Intracellular retention of PCX	−	Increased spreading	−
Rab20	−		−		−
Rab23	−		−		−
Rab24	−	Reduced proliferation	−		−
Rab25	+	Intracellular retention of PCX near the apical site	−		−
Rab27A	+	Intracellular retention of PCX near the apical site	+/−	Slight intracellular retention of PCX	+/−
Rab32	+	Intracellular retention of PCX near the apical site/scattered vesicles	+	Lateral lumens	+
Rab34	−		−		−
Rab35	+	Intracellular retention of PCX near the apical site/scattered vesicles	+	Intracellular retention of PCX in actin-rich clusters	+

Defects in PCX localization are shown by + (clear defect), +/− (mild defect), and − (no defect). Detailed image data are shown in Figs. 4, 5, S4, and S5.