Table 3.

Antibacterial activity of bimetallic nanoparticles against Gram-positive and -negative bacteria

Samples	Staphylococcus aureus		Streptococcus mutans		Escherichia coli		Acinetobacter baumannii
	IC50	MIC	IC50	MIC	IC50	MIC	IC50	MIC
B1	27.94	67.87	25.15	62.61	13.56	49.98	16.89	49.24
B2	34.29	72.39	26.98	68.23	12.89	30.34	20.39	44.12
B3	54.12	98.14	48.67	95.91	23.67	55.87	43.18	85.23
Ampicillin	65.91	>100	26.03	62.24	1.03	2.89	<100	<100
Rifampicin	>100	>100	>100	>100	23.81	86.23	87.12	<100

Notes: The values are in μg/mL. The lowest concentrations of nanoparticle inhibiting ≥90% and 50% with respect to the growth control without nanoparticles were taken as the MIC and IC₅₀, respectively. The experiments were repeated thrice. B1, B2, and B3, gold–silver bimetallic nanoparticles.

Abbreviations: IC₅₀, half maximal inhibitory concentration; MIC, minimum inhibitory concentration.