Cytotoxicity effect of RAMBO, cilengitide, and RAMBO and cilengitide combined on glioma cell lines. Glioma cells grown in 96-well plates were treated for 36 h with various MOIs of RAMBO and for 33 h with various concentrations of cilengitide. Cell viability was determined using the WST-1 assay. For the most part, the higher was the MOI, the greater the reduction in cell survival. Moreover, the addition of cilengitide enhanced the reduction of cell viability. U251 glioma cells (A): Gli36 glioma cells (B): U87ΔEGFR glioma cells(C).