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Example of the metaphase-TIF assay. In this example, RPE-1 hTERT cells were transduced with a TRF2 shRNA to induce TIF, chromosome spreading was prepared using cytocentrifugation, and the sample stained with telomere PNA (green), γ-H2A.X (red) IF, and DAPI (blue). Individual and merged colors of an expanded region are shown on the right.
