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. 2016 May 3;9:2601–2613. doi: 10.2147/OTT.S101209

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© 2016 Lin et al. This work is published and licensed by Dove Medical Press Limited

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Delivery of pSur/AS-Sur downregulates the expression of survivin and XIAP and inhibits the proliferation of cancer cells.

Notes: (A) PANC-1 cells were transfected with either scramble siRNA or survivin siRNA for the indicated durations. Expression of different proteins was determined by Western blotting. The numbers under each blot are the intensities of the blot relative to that of the control (scramble). (B) Cancer cells were transfected with either empty plasmid or pSur/AS-Sur for the indicated durations. The expression of survivin, XIAP, and Bcl-2 were examined by Western blotting. Equal protein loading was verified by actin. The numbers under each blot are the intensities of the blot relative to that of the control (empty plasmid). (C) Cancer cells were transfected with pSur/AS-Sur for 4 days. Cell proliferation was evaluated using the BrdU proliferation assay. “***” denotes statistical significance with a P-value <0.001 between the testing groups. (D) PANC-1 and HUVECs were transfected with either an empty plasmid or pSur/AS-Sur for 4 days. Cytotoxicity was evaluated using the LDH assay. “**” denotes statistical significance with a P-value <0.01 between the testing groups. “NS” denotes no statistical significant difference between the testing groups. (E) Expression of survivin in KB-TAX50 and MCF7-TamC3 cells was determined by Western blotting. (F) Expression of MDR1 in KB and KB-TAX50 cells was determined by RT-PCR. (G) KB-TAX50 and MCF7-TamC3 cells were transfected with pSur/AS-Sur for 4 days. Cell proliferation was evaluated using the BrdU proliferation assay. “***” denotes statistical significance with a P-value <0.001 between the testing groups.

Abbreviations: HUVEC, human umbilical vein endothelial cell; RT-PCR, reverse transcription-polymerase chain reaction; siRNA, small interfering RNA; h, hours; BrdU, Bromodeoxyuridine; XIAP, X-linked inhibitor of apoptosis protein; pSur/AS-Sur, survivin promoter-driven antisense survivin.