Skip to main content
. 2015 Nov 17;108(1):djv303. doi: 10.1093/jnci/djv303

Figure 3.

Figure 3.

miR-34s regulation of PDL1. Endogenous levels of miR-34a, -b, and -c in non–small cell lung cancer (NSCLC) cell lines. A) PDL1 expression in A549, H460, and H1299 cells transfected with miR-34a, -b, and -c. B-J) NSCLC cell lines were treated with miR-34a, miR-34b, or miR-34c at 100nM, and 24 hours later RNA was isolated to study miR-34a, -b, and -c transfection efficiency. At 96 hours after transfection, cell lysates were collected for protein analysis. Quantification of western blots shows that forced overexpression of miR-34a, miR-34b, or miR-34c suppressed PDL1 protein expression compared with a scrambled control. K) Luciferase activity in cells cotransfected with miR-34a, -b, or -c or a scrambled control and a luciferase reporter construct encoding the luciferase gene fused either to the wild-type PDL1 3’ UTR (PDL1 wt) or a mutated PDL1 3’ UTR (PDL1 mut). All three of the miR-34s reduced luciferase activity (P < .001, P =.006, and P =.006). An unpaired t test was used to calculate the two-sided P values. *P < .05, **P < .01, ***P < .001. Error bars on the bar charts represent standard deviation. Similar results were observed in three replicates. mut = mutated; NSCLC = non–small cell lung cancer; scr = scrambled; wt = wild-type.