Figure 4.

TARA protein levels do not cycle in circadian pacemaker neurons. (A) Immunostaining of TARA::GFP in male fly brains on the 3^rd day in DD. We used transgenic flies that carry an artificial exon encoding GFP inserted into an intron of tara in the genome ⁵² and therefore are expected to produce endogenous levels of TARA protein fused to GFP. Brains were dissected at indicated circadian times (CT) and stained for GFP (green) and PDF (red), which was used to identify small ventral lateral neurons (sLN_vs), the pacemaker neurons in DD. Scale bar 10µm. (B) Quantification of TARA::GFP signal in sLN_vs. Data from 11−16 brain hemispheres are presented. Mean ± SEM is shown. ns: not significant, 2-way ANOVA followed by Tukey post hoc test (B).