FIG 6.

Parasite burden versus macrophage viability. Using a visual high-content phenotypic assay, we calculated the percentage of viable macrophages and the percentage of infected cells. We analyzed the antileishmanial effect of the selected compounds from the main library (A), the indirubin library (B), and the purine library (C) versus their toxicity against macrophages. Sectors: a, compounds that are not potent against intracellular parasites but cytotoxic; b, compounds that are not potent against intracellular parasites and not cytotoxic; c, compounds that are potent against intracellular parasites but cytotoxic; and d, compounds that are potent against intracellular parasites and not cytotoxic.