TABLE 1.
IC50s of promastigote and amastigote killing
| Compound | Mean IC50 (μM) for promastigotes ± SDa | Mean IC50 (μM) for amastigotes inside BMDMs ± SDa | Mean IC50 (μM) for amastigotes inside BMDDCs ± SDa | Therapeutic index for BMDMsb | Therapeutic index for BMDDCsb |
|---|---|---|---|---|---|
| Sodium stibogluconate | 251.3 + 15.6 | 14.32 + 1.01 | 47.91 + 6.46 | 10.6 | 4.6 |
| Solamargine | 35.06 + 5.4c | 13.36 + 3.06 | 6.03 + 2.27c | 9.3 | 43.3 |
| Solasonine | 36.5 + 0.26c | 9.30 + 0.59c | 5.93 + 0.96c | 20.2 | 38.3 |
a
The IC50 is the concentration of drug (micromolar) to achieve 50% killing of parasites per 106 axenic promastigotes or 2.5 × 106 amastigotes inside 0.5 × 106 BMDMs or BMDDCs after 48 h of culture.
b
The therapeutic index is the mean 50% cellular cytotoxicity (CC50) of uninfected BMDMs (or BMDDCs)/mean IC50 of amastigotes inside BMDMs (or BMDDCs).
c
Significantly different (P < 0.05) compared to sodium stibogluconate.