TABLE 5.

Potencies of grazoprevir and elbasvir in genotype 1a(H77) cell lines selected with the independent agents or combinations of inhibitors at different concentrations

EC90 multiple used for selectiona		Inhibitor potency
		Elbasvir				Grazoprevir
Grazoprevir	Elbasvir	EC50		EC90		EC50		EC90
		Value, nM	Fold shiftb	Value, nM	Fold shift	Value, nM	Fold shift	Value, nM	Fold shift
0	0	0.002	1	0.003	1	1	1	2	1
0	1×	0.3	150	2.4	800	1	1	2	1
0	10×	0.5	250	2.3	767	1	1	1.2	0.6
0	100×	5	2,500	22	7,333	1	1	2	1
0	1,000×	36	18,000	63	21,000	1	1	2	1
1×	0	0.003	1.5	0.004	1	2	2	10	5
10×	0	NTc	NT	NT	NT	48	48	92	46
1×	1×	0.04	20	1	333	4	4	7	3.5
1×	10×	1.3	650	4.6	1,533	2	2	3	1.5

^aEC₉₀ multiples used for GT1a(H77) selection: grazoprevir EC₉₀, 1.5 nM; elbasvir EC₉₀, 6 pM.

^bChange in potency relative to the DMSO (0×/0×) control.

^cNT, not tested.