TABLE 7.

Potencies of grazoprevir in transiently expressed genotype 1a(H77) replicons bearing NS3 RAVs from resistant colonies

Variant	EC50, nMa	Fold shift vs WTb	Relative fitnessc
WT	0.3 ± 0.1	1	1
R109K	0.6 ± 0.11	2	2.4
D168A	82 ± 12	273	0.5
D168E	11 ± 1.3	37	0.4
D168V	46 ± 20	153	0.3
R155K+D168A	1.9 ± 0.7	6	0.7

^aAverage ± standard deviation; n ≥ 3.

^bChange in potency relative to the WT GT1a(H77) control, rounded up to whole numbers for easy discussion in the text.

^cFitness range: ≤0.02, unfit; 0.03 to 0.5, moderately fit; >0.5, fit.