FIG 2.
Inhibition of systemic infection of MDRAb by the synbiotic. KM, CTM, LOM, MTN, and IPM were administered to mice (18 mice/group) from day −7 until day 28. Mice were infected orally with MDRAb (1 × 104 to 2 × 104 CFU/mouse) on day 0. BbY (1 × 108 to 3 × 108 CFU/mouse/day) and/or GOS (10 mg/mouse/day) was administered orally once daily from day −7 until day 14. On day 4 after the MDRAb infection, animals were injected intraperitoneally with 5-FU at a dose of 400 mg/kg of body weight. Seventy-two mice were dissected 14 days after being infected with MDRAb. Results are expressed as the means ± SD for log10 CFU per gram of mesenteric lymph nodes (MLNs) (a) and livers (b) of mice. The experiment at the same procedure was repeated three times in total. Black bars, saline-treated infected control; white bars, BbY; light gray bars, GOS; dark gray bars, BbY plus GOS. Where indicated, significant differences (**, P < 0.01; ***, P < 0.001) were observed between mice treated with the experimental agents and saline-treated infected control mice. Data are presented as means, error bars indicate SD, and animal numbers are as indicated.