TABLE 1.

Composition and coding parameters of WT and CpG/UpA-low luciferase sequences

Sequence	Symbol	No. of substitutionsa	% C+G content	No. of CpG dinucleotides	ΔCpGb	Ratio of O/E frequencies for CpGc	No. of UpA dinucleotides	ΔUpAb	Ratio of O/E frequencies for UpA	CAId	CPSe
WT	L		0.44	100		1.21	86		0.70	0.719	−0.011
CpG/UpA-low	l	174	0.45	0	100	0	22	64	0.17	0.804	0.021

^aNumber of sequence changes from the WT sequence.

^bChanges in the numbers of CpG and UpA dinucleotides.

^cRatio of observed to expected frequencies of CpG and UpA dinucleotides.

^dCalculated by using the CAIcal website (http://genomes.urv.es/CAIcal/) (40).

^eCodon pair score; calculated as previously described (26, 41).