FIG 5.
Circular alignments of the DNA sequences of four blaNDM-positive plasmids obtained in this study and known related plasmid sequences. The visualized area shows that the percent identity of similar genes between the reference plasmid and other plasmids was at least 80%. The known sequences of the following plasmids (GenBank accession numbers) were included: pNDM-US (NZ_CP006661), pNDM-PstGN576 (KJ802405), pNDM-EcoGN568 (KJ802404), pNDM-US-2 (KJ588779), pKP1-NDM-1 (KF992018), pNDM10469 (JN861072), pNDM-KN (JN157804), pNDM102337 (JF714412), pNDM10505 (JF503991), pNDM-1_Dok01 (AP012208), pKPCAPSS (KP008371), pKpQIL-531 (CP008833), pKpQIL-6e6 (CP007730), pKPN4 (CP000649), and plasmid2 (CP009115). (A) Alignment of replicon type A/C plasmids. Draft genome sequence data for plasmids pV001-a, pV004-a, and pV266-a were obtained in this study. Ten known sequences of blaNDM-positive plasmids were included, and pNDM-1_Dok01 was used as a reference. (B) Alignments of replicon type FIIk plasmid sequences. Draft genome sequence data for plasmid pV308-a were obtained in this study. Six known sequences of blaKPC-2- or blaNDM-1-positive plasmids were included, and pKpQIL-531 (left) and plasmid2 (right) were used as references.