FIG 1.
Fitness defects of MukB-deficient cells. BW25113 (WT), ETBW (ΔtolC), OU140 (ΔmukB), and OU142 (ΔmukB ΔtolC [ΔΔ]) cells were grown at the permissive temperature of 23°C up to an OD600 of 0.6, diluted to OD of 0.05, and further grown at 37°C. (A) Turbidity of the cell culture following temperature upshift. (B) Colony formation (±SD, n = 3) by the cells from panel A before and after growth at 37°C. Note that ΔmukB and ΔmukB ΔtolC cells formed clear colonies by day 2 only. (C and D) MICs for novobiocin (Novo), erythromycin (Ery), norfloxacin (Nor), nalidixic acid (Nal), and rifampin (Rif) for MukB- and TolC-deficient cells at 23°C and (C) and 37°C (D).