FIG 7.

Astrakurkurone differentially regulated phophorylation of p38, ERK1/2, and NF-κB in L. donovani-infected macrophages. A considerable increase of phoshporylated p38 in both uninfected and infected macrophages during the treatment with astrakurkurone has been observed. On the other spectrum, while phosphorylated ERK1/2 was high in infected macrophages, astrakurkurone treatment decreased the phosphorylated ERK1/2 at 15 min. The phosphorylation level of NF-κB after treatment with astrakurkurone also seemed higher than in the untreated infected macrophages. The corresponding histograms show the changes in expression, as measured by densitometry: 1, uninfected; 2, uninfected + treatment; 3, infected; 4, infected + treatment. Data are representative of results of 3 independent experiments.