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. Author manuscript; available in PMC: 2016 May 10.
Published in final edited form as: J Nanomed Nanotechnol. 2016 Jan 25;7(1):350. doi: 10.4172/2157-7439.1000350

Figure 2.

Figure 2

Resveratrol enhances the osteogenic differentiation of hMSCs in 2D culture. (A) Calcium expression was the greatest at all time points for hMSCs cultured in osteogenic medium in the presence of resveratrol. (B) Alizarin Red S staining confirmed enhanced calcium deposition by hMSCs cultured in osteogenic medium with resveratrol at day 21, scale bar is 100 μm. (C) ALP expression levels were the highest for hMSCs cultured in osteogenic medium with resveratrol for each time point, followed by osteogenic medium and basal medium supplemented with resveratrol. (D) ALP staining of hMSCs at day 21 cultured in osteogenic medium with resveratrol, scale bar is 100 μm. (E) qRT-PCR gene expression of hMSCs cultured in basal medium, basal media + 12.5 μM resveratrol, osteogenic medium, and osteogenic medium + 12.5 μM resveratrol. At days 14 and 21, the highest level of BGLAP was detected in hMSCs cultured in osteogenic medium supplemented with resveratrol. (F) Osteocalcin staining of day 21 hMSCs cultured in osteogenic medium + 12.5 μM resveratrol, scale bar is 50 μm. All experiments were done in triplicate. Data points are plotted as mean ± SD (n = 3). A single asterisk denotes significantly (P ≤ 0.05) higher as compared to groups denoted by two asterisks. Two asterisks denotes significantly (P ≤ 0.05) higher as compared to groups denoted by three asterisks.