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. 2016 May 10;11(5):e0155372. doi: 10.1371/journal.pone.0155372

Fig 3. Ush functions in prohemocytes to promote multipotency and block differentiation.

Fig 3

(A-C) Knockdown of Ush (ushRNAi) significantly increased lamellocyte differentiation compared to controls (+). dome-Gal4 females were crossed to control (+) males or males that carry UAS-ushRNAi transgene. Arrow marks lamellocytes (lm). (C) Histogram showing that lamellocyte differentiation was significantly increased in dome-Gal4 driven UAS-ushRNAi lymph glands compared to controls. Fisher’s Exact test; P value is as shown; controls (n = 18); ushRNAi (n = 20). (D-F) Over-expression of Ush in prohemocytes significantly increased the size of the MZ. Tep-Gal4 females were crossed to control (+) males or males that carry the UAS-ush transgene. (F) Histogram showing the size of the MZ significantly increased in lymph glands in which Ush was over-expressed (ushGOF). Student’s t-test; error bars show standard deviation; P values are as shown; control and ushGOF (n = 11). White dotted lines delineate the entire lymph gland; yellow dotted lines delineate the MZ. Scale bars: (A,B) 100 μm; (D,E) 25 μm. (A-C) Lymph glands from late-third instar larvae; (D-F) lymph glands from mid-third instar larvae.