Fig 5. Prohemocyte-specific knockdown of Dorsal limits crystal cell differentiation.

(A,B) Crystal cell numbers were reduced in the lymph glands of dome-Gal4 driven UAS-dl^RNAi (dl^RNAi) compared to controls (+). Lymph glands from mid-third instar larvae. White dotted lines delineate the entire lymph gland. Scale bars: 50 μm. (C) Histogram showing that the percentage of crystal cells decreased in dl^RNAi lymph glands compared to controls. (D) Histogram showing the number of crystal cells decreased in dl^RNAi lymph glands compared to controls. (C,D) Student’s t-test; error bars show standard deviation; control and dl^RNAi (n = 28); P value is as shown.