Fig 6. Dorsal has no effect on Srp expression.

(A-G) Prohemocyte-specific expression of UAS-dl^RNAi, UAS-dl, or UAS-cact^RNAi transgenes. (A,B,G) dome-Gal4 or (C-G) Tep-Gal4 females were crossed to control (+) males or males that carry UAS-dl^RNAi, UAS-dl, or UAS-cact^RNAi transgenes. (A,B) knockdown of Dorsal, (C,D) over-expression of Dorsal or (E,F) knockdown of Cact in prohemocytes has no effect on the level of Srp expression in the lymph glands of mid-third instar larvae. White dotted lines delineate the entire lymph gland. Scale bars: 50 μm. (G) Histogram showing that Srp expression is not significantly different in lymph glands with altered levels of Dorsal or Cact compared to controls. Student’s t-test; for both dome>dl^RNAi and Tep>cact^RNAi and their respective controls (n = 12), control and Tep>dl (n = 13); error bars show standard deviation; P values are as shown.