TABLE 4.
Quantitative proteomic data representing oxidative reactions with HbF Toms River (γV67M)
| Reaction conditions | Asp-67 ratio | Met-67 ratio | Met-67 sulfoxide ratio | Met-67 sulfone ratio | Asp-67 fold change compare with condition 1 |
|---|---|---|---|---|---|
| % | % | % | % | ||
| Patient hemolysate | 7.7 ± 1.4 | 6.0 ± 1.9 | 60.5 ± 3.8 | 25.7 ± 3.7 | |
| 1) CO ligand control | 1.2 ± 0.15 | 51.8 ± 9.74 | 33.48 ± 16.44 | 13.58 ± 6.60 | 1 |
| 2) Control (air equilibration buffer) | 5.0 ± 1.0 | 34.5 ± 7.84 | 49.30 ± 9.18 | 11.24 ± 3.96 | 4.2 |
| 3) 1:1 H2O2 | 5.7 ± 0.3 | 7.18 ± 0.69 | 56.55 ± 0.45 | 30.58 ± 0.47 | 4.8 |
| 4) 2.5:1 H2O2 | 8.8 ± 0.8 | 5.94 ± 0.23 | 52.75 ± 2.25 | 32.66 ± 2.61 | 7.3 |
| 5) 5:1 H2O2 | 15.5 ± 4.2 | 4.03 ± 0.65 | 39.44 ± 3.85 | 41.07 ± 2.73 | 12.9 |
| 6) Ferric/5:1 H2O2 | 2.0 ± 0.4 | 1.21 ± 0.06 | 52.83 ± 0.28 | 43.99 ± 0.53 | 1.6 |