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. 2016 Apr 22;84(5):1642–1649. doi: 10.1128/IAI.01562-15

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FIG 1 — Analysis of pili purified from strains of E. coli expressing cfaABC and cfaABCE. Results of SDS-PAGE (A) and immunoblot (B) analyses of pilus preparations from negative-control strain MC4100 (lane 1), test strain MC4100/pGU1/pHSG576 (cfaABC) (lane 2), and positive-control strain MC4100/pGU1/pGU2 (cfaABCE) (lane 4) are shown. Lane 3 contains molecular size markers. (C, D) Transmission electron microscopy of negatively stained pilus preparations confirmed that although MC4100/pGU1/pGU2 produces CFA/I pili (C), test strain MC4100/pGU1/pHSG576 does not produce pili (D).