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Purification of wild-type and mutant CFA/I pili. Purified pili were analyzed by SDS-PAGE (A) and immunoblotting with anti-CFA/I antiserum (B). Lanes: 1, protein molecular size markers; 2, wild-type pili; 3, pili containing CfaE1 (R181A substitution); 4, pili containing CfaE2 (R67A substitution); 5, pili containing CfaE3 (R67A and R181A substitutions).
