TABLE 1.
Plasmids used in this study
| Plasmid | Backbone vector | Relevant characteristic | Reference/source |
|---|---|---|---|
| pHSG576 | Plac-lacZ′ | 33 | |
| pET22b(+) | PT7, lacI | Novagen | |
| pJGX15W | pJRD184 | Ptet-cfaABCE | 34 |
| pEU2124 | pJRD184 | Ptet-cfaABCE1a | 20 |
| pGU1 | pJRD184 | Ptet-cfaABC | This study |
| pGU2 | pHSG576 | Plac-cfaE | This study |
| pGU3 | pHSG576 | Plac-cfaE1a | This study |
| pGU4 | pHSG576 | Plac-cfaE2b | This study |
| pGU5 | pHSG576 | Plac-cfaE3c | This study |
| pGU6 | pET22b(+) | PT7-dsc19cfaB(His)6 | This study |
| pGU7 | pET22b(+) | PT7-dsc19cfaE(His)6 | This study |
a
R181A mutation in cfaE.
b
R67A mutation in cfaE.
c
R67A R181A double mutation in cfaE.