TABLE 2.

F. tularensis proteins tested for Lon degradation in Lon-deficient E. coli

Gene	Protein function	Abundance ratio from MS dataa	Primer set, plasmidb	Degradationc	Reference(s)d
FTL663	Hypothetical protein	3.786	Pr7261/Pr7262, pST4609	Yes	8
FTL1957	Heat shock protein	2.447	Pr7263/Pr7264, pST4611	Yes	46
FTL578, ocd	Ornithine cyclodeaminase	2.118	Pr7755/Pr7756, pST5007	Yes	64
FTL1217	Hypothetical protein	2.022	Pr7751/Pr7752, pST5009	Yes
FTL1191, dnaK	Molecular chaperone DnaK	1.409	Pr10091/Pr10092, pST6935	No	64
FTL1267	Hypothetical protein	1.350	Pr10095/Pr10096, pST6937	No	7
FTL891, tig	Trigger factor	1.338	Pr10083/Pr10084, pST6931	No	8
FTL1609, flmK	Dolichyl-phosphate-mannose protein	1.145	Pr10099/Pr10100, pST6939	No	7
FTL1228, sufD	SufS activator complex, SufD subunit	1.059	Pr10093/Pr10094, pST6936	Yes	6
FTL893, clpX	ClpX	1.028	Pr7522/Pr7523, pST4819	No	8
FTL1017, cmk	Cytidylate kinase	1.011	Pr7518/Pr7519, pST4818	No
FTL478, gcvH	Glycine cleavage system protein H	1.000	Pr7516/Pr7517, pST4816	No	7
FTL398, purE	Phosphoribosylaminoimidazole carboxylase, catalytic subunit	0.983	Pr10077/Pr10078, pST6928	No	7
FTL1358	Cation efflux family protein	0.957	Pr10097/Pr10098, pST6938	No	7
FTL1611, yfdH	Glycosyl transferase family protein	0.932	Pr9901/Pr9902, pST7048	No	7
FTL1724	Lipoprotein	0.887	Pr9903/Pr9904, pST7049	No	7
FTL87	Acetyltransferase	0.881	Pr9891/Pr9892, pST7043	No	77
FTL40	LysR family transcriptional regulator	0.856	Pr10075/Pr10076, pST6927	No	6
FTL599, wbtG	Glycosyl transferase family protein	0.838	Pr9897/Pr9898 pST7046	No	7
FTL687, emrA1	HlyD family secretion protein	0.814	Pr10081/Pr10082 pST6930	No	7
FTL593	Galactosyl transferase	0.797	Pr9893/Pr9894 pST7044	No	78
FTL595, wbtE	Galacturonosyl transferase	0.794	Pr9895/Pr9896 pST7045	No	7, 79

^aRatio of abundance of the protein in the Δlon mutant to that in the wild-type strains.

^bPrimer set used to amplify each target gene and pACYCDuet derivative containing the target gene.

^cDegradable or undegradable by the F. tularensis Lon protease in E. coli.

^dPrevious characterization if available.