FIG 2.

Native BdRON2 localizes to the apical complex. Immunofluorescence staining of anti-BdRON2_503–602 antibodies (Bd₅₀₃) and anti-BbRON2_peptide antibodies (Bb_PEP) with fluorescein isothiocyanate (FITC)-conjugated anti-rabbit immunoglobulin secondary antibodies on fixed red blood cells infected with B. divergens parasites shows that native BdRON2 is localized mainly at the apical ends of the intracellular parasites. Staining with DAPI in mounting medium identifies the nucleus of the parasite. Phase-contrast microscopy images confirm the location and number of individual intracellular parasites. Bars, 5 μm.