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. 2016 Apr 22;84(5):1574–1584. doi: 10.1128/IAI.00107-16

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FIG 5 — B. microti RON2 is an ∼170-kDa antigen. (A) Immunoblotting with B. microti-infected lysates from mouse RBCs and anti-BdRON2_503–602 antibodies (lane Bd₅₀₃) detected a doublet, with the predominant band being of the predicted size of ∼170 kDa (arrow). (B) Blotting with anti-BbRON2_peptide antibodies (lane Bb_PEP) also detected the predominant band of ∼170 kDa (arrow) and a product of ∼55 kDa. Preimmune rabbit serum (lanes PI) did not react with any native antigens. Numbers to the left of the gels are molecular masses (in kilodaltons).