FIG 1.

C57BL/6 RAG1^−/− mice develop only mild disease and survive the initial phase of R. typhi infection. (A) C57BL/6 RAG1^−/− mice were infected with 2 × 10⁶ SFU R. typhi either i.v. (n = 13) or s.c. (n = 16). The course of disease was evaluated by clinical scoring (left, y axis) and quantification of serum GPT levels (right, y axis) at the indicated points in time postinfection (x axis). All mice survived. (B) C57BL/6 wild-type (open symbols, n = 5 to 7) and C57BL/6 RAG1^−/− mice (black symbols, n = 5 to 7) were infected with 2 × 10⁶ SFU R. typhi s.c., and plasma cytokine levels (y axes) were quantified with the bead-based LEGENDplex assay at the indicated points in time postinfection (x axes). Serum levels of IFN-γ, IL-6, TNF-α, IL-12p70, and MCP-1 are depicted. Combined results from two independent experiments are shown. Error bars show standard error of the mean (SEM). Statistical analysis was performed with the two-tailed Mann-Whitney U test. Asterisks indicate statistically significant differences compared to naive mice (day 0) (*, P < 0.05; **, P < 0.01).