FIG 2.

Myosin deletion strains are sensitive to anti-cell wall agents. (A) Deletion of myoB resulted in increased sensitivity to the β-(1,3)-glucan synthase inhibitor caspofungin (CSP) (1 μg/ml) but did not abolish paradoxical growth at 4 μg/ml CSP on plates. Deletion of both myoB and myoE resulted in increased sensitivity to both CSP and the chitin synthase inhibitor nikkomycin Z (NikZ). Conidia (10⁴) were grown on GMM supplemented with the appropriate anti-cell wall drugs for 5 days at 37°C. (B) In liquid medium, deletion of myoB or myoE resulted in increased sensitivity to caspofungin, but only the ΔmyoB strain exhibited loss of paradoxical growth. The MEC of caspofungin for the ΔmyoB strain was 0.25 μg/ml and for the ΔmyoE strain was 0.125 μg/ml. Conidia were grown in RPMI supplemented with the appropriate concentrations of caspofungin for 48 h at 37°C.