FIG 2.

PNPase deficiency leads to a reduced growth rate. Wild-type FAM20 and the Δpnp mutant were inoculated to an OD₆₀₀ of 0.1 and were grown in GC medium supplemented with 1% Kellogg's solution at 37°C under 5% CO₂ with shaking. Growth was monitored for 24 h by measuring the OD at 600 nm.