TABLE 2.
Primers used in this study
| Primer | Sequence |
|---|---|
| Cloning | |
| P1 | ATAGAAAAGTTGAGCGATTCAGCTTTATAC |
| P2 | TGTACAAACTTGAATACCGCACTGCTAAAAC |
| P3 | TGTACAAAGTGGCGCTTAGGGTGAAAGTGC |
| P4 | ATAATAAAGTTGGATACTCGGATTAATCGG |
| P5 | ATAGAAAAGTTGATGCCGTCTGAAAATTAAGTTAGAATTATCCC |
| P6 | GGGGACTGCTTTTTTGTACAAACTTGAAATCCAAAATCATACTG |
| P7 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGACTTTGCTGACTCAGGATT |
| P8 | GGGGACCACTTTGTACAAGAAAGCTGGGTAAATACCGTCTGAAACCTG |
| K1 | AAAAAGCAGGCTGTATTAGTGACCTGTAGAATTCGAGC |
| K2 | AGAAAGCTGGGTTTAGAAAAACTCATCGAGCATCAAATG |
| Lac-pnpfw | TAACAATTTCACACAGGAAACAGCTTTGAAAGGAACAATAATGTTCAATAA |
| Pnp-down | CATTCATTCCCGATACCCGTAAAAA |
| Lac_up | TCCCCATCGGTGATGTCGTATAAGA |
| Lac-PNPrev | TTATTGAACATTATTGTTCCTTTCAAAGCTGTTTCCTGTGTGAAATTGTTA |
| PilEUHSfw | ATAGAAAAGTTGAGGCGGATGTGTAAGGTAGATG |
| PilEUHSrev | TGTACAAACTTGCGATCAGGGTGAAACCTT |
| PilEDHSfw | CTTGTACAAAGTGGACCTGCCGCACCAAATAA |
| PilEDHSrev | ATAATAAAGTTGGCAGGGAAATGGGTAGGGA |
| attB1_Tet_fw | AAAAAGCAGGCTACCTTTCCGAACAAGA |
| attB2_Tet_rev | CAAGAAAGCTGGGTTTCAGACGGCATTGGGT |
| TetRfw | TTTTTTTCAAACGAAGTCAGCCCCATACGATATAAG |
| TetRrev | TTCCATTCAGGTCGAGGTGGCC |
| NMC50fw | ATGTCAATCAATACGTTTGAAACCTTTATTTACG |
| NMC50rev | GGGCTGACTTCGTTTGAAAAAAAAGCGTG |
| SiaCfw | ATGCCGTCTGAATATGAACGATTTATCTGAAGC |
| SiaCrev | GATGAATAAGACTCCAGCTTAAATATATTTATTCAATATCAGTTTTTTTG |
| NMC49fw | ACCTGAATGGAATCAACTATTTCTTCTTCTAAACCATTTAG |
| NMC49rev | TTGACAATAGAGCGAAAAAGTACCGCG |
| SiaE-Tetfw | ATATATTTAAGCTGGAGTCTTATTCATCATGTCAATCAATACGTTTG |
| SiaE-Tetrev | AGAAATAGTTGATTCCATTCAGGTCGAGGTG |
| PNPpurfw | TTGAAAGGAACAATAATGTTCAATAAACAC |
| PNPpurrev | TTACTCGGCGGCATTTTC |
| qPCR | |
| PNP_qPCR_fw | TTGGGCGACGAAGACCACTT |
| PNP_qPCR_rev | TGTGCAGACGCGCTTCTTTG |
| S10_qPCR_fw | TTGGAAATCCGCACCCACTT |
| S10_qPCR_rev | TACATCAACACCGGCCGACAAA |
| PilN_qPCR_fw | GAGATGAACAAGCGCAAACA |
| PilN_qPCR_rev | AAGTGTGCGATGGAGGTTTC |
| PilO_qPCR_fw | CAGATGGAATCCCTTGAGGA |
| PilO_qPCR_rev | GAACCTGCCTGATGAAGCTC |
| PilW_qPCR_fw | AACTACGGCTGGTTCCTGTG |
| PilW_qPCR_rev | GTGCGCGCCAGTTCTTTAAA |