TABLE 1.
Strains and primers in this study
| Strain or primer | Description or sequencea | Source |
|---|---|---|
| S. sanguinis strains | ||
| SK36 | Human plaque isolate | Kilian et al. (37) |
| Δnox | Kanr; ΔSSA_1127::aphA-3 | This study |
| Δnox_compl | Ermr; ΔSSA_1127::erm | This study |
| ΔspxB | Kanr; ΔSSA_0391::aphA-3 | Chen et al. (17) |
| JFP36 | Ermr; ΔSSA_0169::pSerm | Turner et al. (25) |
| Primers | ||
| nox_F1 | CCATCTACCGACTTGTCTGAAAC | |
| nox_R1 | GCCATTTATTCCTCCTAGTTAGTCAACTCATAAGAATAGTCCTACCTTA | |
| Kan_F2 | TGACTAACTAGGAGGAATAAATGGCTAAAATGAGAATAT | |
| Kan_R2 | CATTATTCCCTCCAGGTACTAAAACAATTCATCCAGT | |
| nox_F3 | GTTTTAGTACCTGGAGGGAATAATGATTACTCAAGCAGCTTTGAAAGC | |
| nox_R3 | GTAGGAAATAACCAATCGGAAGAAT | |
| nox_compl_F1 | nox_F1 | |
| nox_compl_R1 | TGTAATCACTCCTTCTCACTATTTATTTTGCTTTCAAAGCTGCTTGA | |
| Erm_F2 | TAAATAGTGAGAAGGAGTGATTACATGAACAA | |
| Erm_R2 | TTATTTCCTCCCGTTAAATAATAG | |
| nox_compl_F3 | CTATTATTTAACGGGAGGAAATAAGAAAATGAGTCTGGGATAAATTTCCA | |
| nox_compl_R3 | nox_R3 | |
| nox_rp_F | GACGACGACAAGATCAGTAAAATCGTTGTAGTTGGTGCAA | |
| nox_rp_R | GAGGAGAAGCCCGGTTATTTTGCTTTCAAAGCTGCTTGA | |
| spxB_L | ATCACTCAACACCGTCCACTTCCA | |
| spxB_R | TCTTCCAAGAAGAGGCGGAATGGT | |
| gyrA_L | AGCTGATTGCCTTGATTGCAGAC | |
| gyrA_R | ATCCGCAAATTTACGCTTGACCT |
a
Kan, kanamycin; Erm, erythromycin.