FIG 8.

Predicting in vivo attenuations from in vitro M. tuberculosis growth rates. We implemented the paradigm described in Fig. 1 by correlating CBM-predicted growth rates and average GranSim-CBM-predicted CFU per granuloma. We plotted CBM-predicted growth rates for single gene knockouts in rich media (A) or lipid-only media (B and C) versus average GranSim-CBM predicted CFU per granuloma when knocked out from the start of infection (A and B) or knocked out mid-infection at 200 dpi (C). Scatter plot of means ± standard errors of the means (SEM) (n = 20) for each knockout and wild type. Note that the high-CFU-burden knockouts can be cleanly separated from the low-CFU-burden knockouts on the basis of the CBM growth rate in lipid-only media but that the timing of the knockout moves the in vitro threshold to the left (arrow in panel C).