FIG 1.

Schematic of the workflow used to identify Bcc adhesins from bacterial membrane proteins. The membrane proteins were analyzed by using 2D gels, and pairs of gels were either blotted onto PVDF membranes or stained with Coomassie blue. The blots were probed with lung epithelial cells, followed by detection of bound cells by antibodies. Positive spots on exposed chemiluminescent films were matched to the paired Coomassie blue-stained gels, and the proteins in the corresponding gel-based spots were excised, trypsin digested, and identified by MALDI-TOF MS analysis.