TABLE 2.
Proteins involved in attachment to lung epithelial cells identified from cell blots probed with CFBE41o− or 16HBE14o− cells
| Protein | BCA designation, alias | Functional category | Predicted subcellular locationa (score) | Strain(s) in which protein was identified | Molecular mass (kDa)/pIb | Sequence coverage (%) | Scorec |
|---|---|---|---|---|---|---|---|
| Linocin_M18 bacteriocin protein | Bcen1404 | Encapsulating protein | Cytoplasmic (8.96) | B. cenocepacia BC7 and C1394; B. multivorans C1962 and LMG13010 | 29.0/4.8 | 66–85 | 87–215 |
| OmpW family protein | L1829 | Membrane protein | Outer membrane (9.45) | B. cenocepacia BC7 and C1394; B. multivorans C1962 and LMG13010 | 22.9/7.8 | 73.2–85.9 | 84–143 |
| Alkyl hydroperoxide reductase | L2013 | Oxidoreductase | Cytoplasmic (997) | B. cenocepacia BC7 and C1394; B. multivorans LMG13010 | 20.5/4.9 | 57.1–65.9 | 115–137 |
| Histone family protein DNA-binding protein | M1012 | DNA binding | Unknown (2) | B. cenocepacia BC7 and C1394; B. multivorans LMG13010 | 15.2/11.2 | 46.9–49.7 | 81 |
| Acetoacetyl-CoA reductase | L0833, PhbB | Oxidoreductase | Cytoplasmic (9.26) | B. cenocepacia C1394; B. multivorans LMG13010 | 28.4/7.7 | 53–63 | 90–209 |
| Hcp1 family type VI secretion system effector | L0343, BcsL | Secretion | Extracellular (10) | B. cenocepacia C1394; B. multivorans C1962 | 18.5/6.9 | 52.1–88 | 97–161 |
| Heat shock protein Hsp20 | Bmul5914 | Stress response | Cytoplasmic (8.96) | B. cenocepacia BC7; B. multivorans LMG13010 | 21.4/5.2 | 41.4–52.9 | 96–104 |
| Hypothetical protein Bmul_4930 | M0843 | Lipoproteind | Transmembrane | B. cenocepacia BC7; B. multivorans C1962 | 19.6/10.3 | 58.2–82 | 124 |
| OmpA/MotB domain-containing protein | L2645 | Membrane protein | Outer membrane (9.45) | B. cenocepacia C1394; B. multivorans C1962 | 21.5/10.4 | 42 | |
| Oxidoreductase, aldo/keto reductase family | L0513 | Oxidoreductase | Cytoplasmic (9.97) | B. cenocepacia BC7 | 38.2/5.8 | 69.5 | 246 |
| Putative universal stress protein | BURMUCGD2_RS23075 | Stress response | Cytoplasmic (8.96) | B. cenocepacia C1394; B. multivorans C1962 | 17.6/6.1 | 70.4–95.1 | 196–285 |
| Hypothetical protein Bmul_4929 (also known as protein of unknown function, DUF883, ElaB) | M0844 | Membrane proteind | Membraned | B. multivorans C1962 | 13.9/7.8 | 78.9 | 85 |
| Type VI secretion protein (97% homology between both GIs) | L0341 | Secretion | Cytoplasmic (9.97) | B. cenocepacia BC7 | 19.2/5.1–5.3 | 60.8 | 97–112 |
| Ubiquinone/menaquinone biosynthesis methyltransferase | L0873, UbiE | Methyltransferase | Cytoplasmic (9.96) | B. cenocepacia C1394 | 27.1/9.1 | 67.9–77.4 | 95–210 |
a
Predicted subcellular localization determined by using PSORTb V3 (http://www.psort.org/) (63).
b
Theoretical molecular masses (kilodaltons) and isoelectric points were determined by Mascot analysis.
c
Ranges represent MS/MS ion scores determined by peptide mass fingerprinting (64). Only scores that were deemed to be significant by Mascot analysis (P < 0.05) are reported.
d
Functional category from UniProt and/or the Burkholderia Genome Database (42).