TABLE 3.
Identification of linocin and OmpW from 2D blots probed with sera from Bcc-colonized CF patients compared with control blots using sera from CF patients with no history of Bcc infection
| GI sequence identifier | Protein (species) | Molecular mass (kDa)/pIa | Strain | Sequence coverage (%) | Scoreb |
|---|---|---|---|---|---|
| GI 161524680 | OmpW family protein | 22.9/9 | BC7 | 27–85 | 54–142 |
| C1394 | 10 | 74–78 | |||
| LMG10303 | 29–85 | 108–253 | |||
| C1962 | 36–84 | 43–222 | |||
| GI 221198593 | Linocin (B. multivorans) | 28.8/4.8 | BC7 | 73.8–78.2 | 155–572 |
| GI 170735569 | Linocin (B. cenocepacia)c | 28.8/4.8 | C1394 | 41.7–50.9 | 76–89 |
| GI 221198593 | Linocin (B. multivorans) | 28.8/4.8 | LMG13010 | 34–81 | 193–488 |
| GI 221198593 | Linocin (B. multivorans) | 28.8/4.8 | C1962 | 48.3–90 | 105–141 |
a
Theoretical molecular masses (kilodaltons) and isoelectric points were determined by Mascot analysis.
b
Ranges represent MS/MS ion scores determined by mass fingerprinting. Only scores that were deemed to be significant by Mascot analysis (P < 0.05) are reported.
c
Query coverage of 96%; 92% identity.