FIG 4.

(A) Acid survival of C. jejuni mutants relative to the wild-type strain. The C. jejuni Δrrc, Δcj0818, ΔflgR, ΔflgP, ΔflhB, ΔflgD, ΔflgK, ΔflgH, and ΔmotAB mutants but not the ΔflgE2 mutant were more sensitive to acid than the wild-type strain. (B) Acid-sensitive C. jejuni mutants were defective in motility on soft MH agar compared to the motility of the wild-type strain. Results are represented as the mean diameters of bacterial migration from the site of inoculation ± standard errors for three biological experiments each with three technical replicates. Data from a minimum of three independent experiments are shown as the mean ± standard error. P values were determined using ANOVA followed by a Bonferroni multiple-comparison test.