TABLE 2.
Primer used in this study
| Primer purpose and name | Primer sequencea (5′-3′) |
|---|---|
| RT-qPCR | |
| Cj0414F (+) | CTGTTTTAAAGGCAGCAGAACTTAC |
| Cj0414R (−) | CTTCTCCTTGCTCATCTTTAGG |
| Cj0415F (+) | GCTTTAGGTTCTATGGTGGCTTT |
| Cj0415R (−) | AAAAGTGGATCACCCCAAGA |
| Cj0448cF (+) | GGAACATTGCATAGAAGTGTAGATG |
| Cj0448cR (−) | CTAGTTTTCTTACTTCATCGGCAAC |
| clpBF (+) | AAGCCGTACGAAGAAAACCTTATAG |
| clpBR (−) | AATCCACTGTTACACCTTTGCTATC |
| flaBF (−) | CGAACCAATGTCTGCTCTGA |
| flaBF (+) | GCAGGCTCAGGTTTTTCAG |
| grpEF (+) | GCTTTAGAAGCAGCTGTTAATG |
| grpER (−) | CATCTTTGATAAGAGCCACTCC |
| katAF (+) | CTTTAGTCCAAGCAATATCGTTCC |
| katAR (−) | CAGCGACATTGTAAGTATTCACTTC |
| metBF (+) | AAACTTTAGCATTACACGGAGCTT |
| metCF (+) | CTAAACTTATTCATTGTGGCAGAGG |
| metCR (−) | CTCTGTATTTTTCCAAGTTGCGTG |
| metBR (−) | CCCTCCTTCGACATTAGCAA |
| rpsLF (+) | CAAAGAAGGGGAGTTTGCAC |
| rpsLR (−) | TATCAAGAGCACCACGAACG |
| chuA (+) | TGCTTTCAAAATTCATTTTTATCC |
| chuA (−) | TTGGGTGCAAATTTTACTCCTT |
| cfrA (+) | TTGCGCCATTGGTCTCTTA |
| cfrA (−) | GCAACATCTCTATAAGGCTTACTT |
| slyD (+) | TACGATGAAAATGCCGTTCA |
| slyD (−) | TTCGCCAAAAAGCTCCATAC |
| Directed mutagenesis (generation of furΔS2 mutant) | |
| furΔS2H43A(F) | AAAACTCTTTATCACAGTGATACTGCCTACACACCCGAAAGTTTATATATG |
| furΔS2H43A(R) | CATATATAAACTTTCGGGTGTGTAGGCAGTATCACTGTGATAAAGAGTTTT |
| furΔS2H102A(F) | CTTGCCAATAAACCTCACCATGATGCCATGATATGTAAAAATTGCGGAAAA |
| furΔS2H102A(R) | TTTTCCGCAATTTTTACATATCATGGCATCATGGTGAGGTTTATTGGCAAG |
| Transformation of furΔS2 into C. jejuni Δfur | |
| JBCL-Fur4 | GATTTAGATGTCTAGACTCAAAAAGGGGAGTGATATGCTGATAGAAAATGTGGA |
| JBCL-Fur2R | GGGGAAGCTTTCTAGTTATATTTTTACCTTTGCTT |
| AR55 | ATGACATTGCCTTCTGCGT |
| EMSAs | |
| JFC1584 | Cy5-TGCATTTTATTGATAATAAATTTCAAAATAAATTTAGTTT |
| JFC1585 | Cy5-AAACTAAATTTATTTTGAAATTTATTATCAATAAAATGCA |
a
Underlined sequences represent mutated nucleotides.