Skip to main content
. Author manuscript; available in PMC: 2017 Apr 11.
Published in final edited form as: Biomacromolecules. 2016 Mar 22;17(4):1477–1485. doi: 10.1021/acs.biomac.6b00082

Figure 3.

Figure 3

Enzyme SA toward HACoA analogs 1a–1h at 1.6 mM. (A) wt- (solid bar) and A479S-PhaCCs (patterned bar). The enzyme concentrations are (wt/A479S) 2.0/2.4 nM, 2.0/2.4 nM, 234/285 nM, 703/854 nM, 703/854 nM, 47/57 nM, 7.8/9.5 nM, and 7.8/9.5 nM for HBCoA (Me), HVCoA (Et), HHxCoA (Pr), HCCoA (pent), HPBCoA (benzyl), HHxeCoA (allyl), HHxyCoA (propynyl), and HABCoA (CH2N3), respectively. (B) PhaCCc. The enzyme concentrations are 81.5 nM, 2.17 µM, 65.21 µM, 32.60 µM, 32.60 µM, 32.60 µM, 32.60 µM, and 32.60 µM for HBCoA, HVCoA, HHxCoA, HCCoA, HPBCoA, HHxeCoA, HHxyCoA, and HABCoA, respectively. (C) PhaECAv. The enzyme concentrations are 5.1 nM, 12.7 nM, 509 nM, 10.17 µM, 10.17 µM, 63.6 nM, 254 nM, 50.9 nM for HBCoA, HVCoA, HHxCoA, HCCoA, HPBCoA, HHxeCoA, HHxyCoA, and HABCoA, respectively.