Table 3.
JTK_Cycle Statistics and Two-factor ANOVA Results. All qPCR data were tested for circadian rhythmicity with JTK_cycle test and two-way ANOVA for repeated measures and a Tukey’s post hoc test. P-values from these tests are summarized.
| Figure | Genotype | Gene | Tissue | JTK_cycle P-value | Time P-value |
Genotype P-value |
Time X Genotype P-value |
|---|---|---|---|---|---|---|---|
| 1C | [ZT] UAS-CLK∆/+ | per | Fat Body (FB) | 0.0194 | < 0.0001 | 0.7826 | 0.4679 |
| 1C | [ZT] Pdf-GAL4/UAS-CLK∆ | per | FB | 0.0094 | |||
| 1D | [CT] UAS-CLK∆/+ | per | FB | 0.0041 | 0.0313 | 0.6650 | 0.0281 |
| 1D | [CT] Pdf-GAL4/UAS-CLK∆ | per | FB | 1 | |||
| 2A | Iso31 | sxe2 | FB | 0.0014 | 0.0131 | 0.1852 | 0.0843 |
| 2A | Clkjrk | sxe2 | FB | 1 | |||
| 2B | Iso31 | CG17562 | FB | 0.0014 | 0.0223 | 0.6409 | 0.1746 |
| 2B | Clkjrk | CG17562 | FB | 0.6945 | |||
| 2C | Iso31 | sxe1 | FB | 0.0020 | 0.0019 | <0.0001 | 0.0020 |
| 2C | Clkjrk | sxe1 | FB | 1 | |||
| 2D | Iso31 | CG14934 | FB | 0.0820 | 0.1146 | <0.0001 | 0.0266 |
| 2D | Clkjrk | CG14934 | FB | 0.5429 | |||
| 3A | UAS-CLK∆/+ | sxe1 | FB | 0.0007 | 0.0003 | 0.0049 | 0.7061 |
| 3A | Pdf-GAL4/UAS-CLK∆ | sxe1 | FB | 9.00E-07 | |||
| 3B | UAS-CYC∆/+ | sxe1 | FB | 0.0363 | 0.0006 | 0.7995 | 0.9592 |
| 3B | 911-GAL4/UAS-CYC∆ | sxe1 | FB | 0.0044 | |||
| 3C | UAS-CLK∆/+ | sxe1 | FB | 0.0014 | 0.0060 | <0.0001 | 0.0749 |
| 3C | Npf-GAL4/UAS-CLK∆ | sxe1 | FB | 0.1969 | |||
| 3D | UAS-CYC∆/+ | sxe1 | FB | 0.0029 | <0.0001 | <0.0001 | <0.0001 |
| 3D | Npf-GAL4/UAS-CYC∆ | sxe1 | FB | 1 | |||
| 3E | UAS-CLK∆/+ | sxe1 | FB | 0.0196 | 0.0038 | 0.0017 | 0.5326 |
| 3E | Dvpdf-GAL4/UAS-CLK∆;Pdf-GAL80/+ | sxe1 | FB | 0.0001 | |||
| 3F | UAS-CLK∆/+ | Cyp6a21 | FB | 0.0009 | <0.0001 | <0.0001 | 0.0574 |
| 3F | Npf-GAL4/UAS-CLK∆ | Cyp6a21 | FB | 0.0259 | |||
| 3G | UAS-CLK∆/+ | per | FB | 0.0568 | 0.0057 | 0.8767 | 0.9902 |
| 3G | Npf-GAL4/UAS-CLK∆ | per | FB | 0.0441 | |||
| 4A | UAS-Npf RNAi/+ | sxe1 | FB | 0.0020 | 0.0005 | <0.0001 | 0.1421 |
| 4A | Npf-GAL4/UAS-Npf RNAi | sxe1 | FB | 0.0441 | |||
| 4B | Not analyzed with JTK | npf | Head | - | |||
| 4C | npfr/+ | sxe1 | FB | 0.0128 | 0.0008 | <0.0001 | 0.0038 |
| 4C | npfr | sxe1 | FB | 0.1969 | |||
| 4D | npfr/+ | Cyp6a21 | FB | 0.0916 | 0.0319 | <0.0001 | 0.4182 |
| 4D | npfr | Cyp6a21 | FB | 0.1150 | |||
| 4E | UAS-CLK∆/+ | npf | Head | 1 | 0.7588 | <0.0001 | 0.846 |
| 4E | Npf-GAL4/UAS-CLK∆ | npf | Head | 1 | |||
| 4F | UAS-CLK∆/+ | per | Head | 0.0001 | <0.0001 | 0.1410 | 0.0420 |
| 4F | Npf-GAL4/UAS-CLK∆ | per | Head | 1.19E-05 | |||
| 5A | Wild type | Cyp2b10 | Liver | 0.0010 | 0.0209 | 0.0034 | 0.1640 |
| 5A | Npy Knockout (KO) | Cyp2b10 | Liver | 0.0510 | |||
| 5B | Wild type | Reverbα | Liver | 7.46E-12 | <0.0001 | 0.4979 | 0.0661 |
| 5B | Npy KO | Reverbα | Liver | 7.17E-09 | |||
| 5C | Wild type | Alas1 | Liver | 9.81E-06 | 0.0007 | 0.8476 | 0.8202 |
| 5C | Npy KO | Alas1 | Liver | 0.0018 |