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. Author manuscript; available in PMC: 2017 Apr 29.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Mar 21;473(2):524–529. doi: 10.1016/j.bbrc.2016.03.096

Fig. 3.

Fig. 3

Probing the levels of core fucosylation on the two forms of 1918 H1N1 neuraminidase through in vitro fucosylation. In all experiments, equal amounts of dimer and tetramer were used for comparison. (A) The tetramer and dimer were fucosylated with azido-fucose by FUT8 and FUT11. The substrate specificity of FUT11 was unknown and used as a negative control. The incorporated azido-fucose was probed with streptavidin-HRP via click chemistry reaction. (B) Fucosylation of the dimer and tetramer with 3H-fucose by FUT8. The samples were spotted on glass fiber filters and counted with a liquid scintillation counter.