Figure 2.

Effect of TF mutations on rates of FX activation by the memTF/FVIIa complex in solution (with 0.1% Triton X-100), measured using different divalent metal ion concentrations. (A) FX activation by the memTF/FVIIa complex using 1.85 mM Ca²⁺ alone, graphed as initial rates of FX activation divided by the memTF/FVIIa complex concentration. (B) Relative rates of FX activation by memTF/FVIIa in solution using 1.25 mM Ca²⁺ and 0.6 mM Mg²⁺, normalized to rates using 1.85 mM Ca²⁺ alone. (C) Relative rates of FX activation by the memTF/FVIIa complex in solution using 1.25 mM Ca²⁺ and 0.6 mM Mg²⁺, normalized to rates using 1.25 mM Ca²⁺ alone. Data are means ± the standard error (n ≥ 3).