Figure 5.

Sir2 is required for ethanol preference and for reward in the mushroom bodies. A, CAFE assay comparing the preference for liquid food with or without ethanol, with the schematic depicting the experimental design (n = 27–28). B, Bitter taste aversion in flies given the choice of sucrose with and without quinine. A value of “−1” indicates complete aversion (n = 7). C, Conditioned olfactory preference to measure ethanol reward, with a schematic diagram of the experimental design in Sir2 mutants and flies expressing Sir2 RNAi under the control of the mushroom body driver 17D-GAL4 (n = 14–16). D, Preference for the odorants isoamyl alcohol and ethyl acetate in the absence of pairing with ethanol inebriation (n = 8). Error bars indicate the SE. *p < 0.05, **p < 0.01.