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. Author manuscript; available in PMC: 2016 Oct 19.
Published in final edited form as: Anal Chem. 2016 Apr 1;88(8):4269–4276. doi: 10.1021/acs.analchem.5b04127

Figure 4. Characterization of Akt and Akt-PH binding to PIP3 lipid vesicles.

Figure 4

(a,b) Quantification of Akt pull-down by various lipid vesicles. Error bars: SD of data from 10 or more imaging areas. (c) Colocalization of Akt and Akt-PH (GFP) with PIP3 vesicles (DiD) from the same region or different regions is shown as percentage of GFP spots that overlapped with DiD spots. (d) Binding of Akt and Akt-PH to PIP3 vesicles over incubation time is shown as fraction of bound vesicles. (e) GFP photobleaching traces of 1 and 2 copies of Akt bound to PIP3. (f) Distribution of Akt stably bound on PIP3 vesicles at various NGFP over incubation time. (g) GFP photobleaching traces of 1, 3, and 4 copies of Akt-PH bound to PIP3. (h) Distribution of Akt-PH stably bound on PIP3 vesicles at various NGFP over incubation time.